Furthermore, chilling/equilibration can be reduced with little impact on sperm characteristics.Īnimal protein-free Equilibration OptiXcell Rhinoceros Semen cryopreservation.Ĭopyright © 2019 Elsevier Inc. OptiXcell is comparable to egg yolk-based EQ when used for rhinoceros semen cryopreservation. Morphology did not differ between fresh (75.0 ± 4.9% normal) and any treatment group (70.0-77.8%) or over time (P > 0.05). They are the most ferocious of all species. i dont really know how else to describe the situation (i am an. But still, they can often surpass 40 to 50 km/h (25 to 30 mph). I have built a model using block x-reffed in from smaller files, so my model is pretty small, about 8 or 9 megs, but my model is moving excruciatingly slow despite this as i try to navigate the work space. Their massive weight has caused them to slow down than their other cousins. Post-thaw acrosomal integrity was lower in EQ, FEQ, and IEQ (56.9 ± 0.7 56.6 ± 4.5 54.9 ± 2.9%) than OP, FOP, IOP (71.8 ± 4.7 71.9 ± 3.8 69.9 ± 4.5%) and fresh (72.6 ± 1.4% P ≤ 0.05). They are the biggest of all species and are found throughout the deserts of Africa.
Motility decreased post-thaw (EQ: 50.7 ± 5.2% OP: 52.9 ± 3.4%) from fresh (82.9 ± 2.9%), was higher in OP than IOP (38.6 ± 4.9% P ≤ 0.05) and decreased over time (P ≤ 0.05). The tentative recovery of Africa’s black rhino population was hailed by conservationists on Thursday as a cause for hopes that ambitious protection efforts could overcome the 'acute threat of poaching. Treatments were diluted with extender and cooled for 15 min (fast: FEQ FOP) or not cooled (immediate: IEQ IOP), prior to cryopreservation. Controls were diluted with equine extender (EQ) or OP and equilibrated for 1 h. Semen was collected from three rhinoceros species: black (Diceros bicornis n = 2), white (Ceratotherium simum n = 2), and greater one-horned (GOH Rhinoceros unicornis n = 3). OptiXcell (OP) was tested as an animal protein-free alternative to an egg yolk-based extender for rhinoceros semen cryopreservation and shorter chilling/equilibration periods were evaluated.
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